THE ALTERATIONS OF THE ENZYMATIC ANTIOXYDANT ACTIVITY BY ADDING ALKALINE WATER ON THE WHITE LABORATORY RATS AFTER HYPERTHERMIC STRESS

Majlinda Ademi, Icko Gjorgoski, Ilbert Ademi

Abstract

Alkaline water is in the focus of scientific interest over the last decade, due to its ability to alkalize the organism as well as its antioxidant effect. Due the hyperthermia, systemic and cellular changes occurs. Adding alkaline water (AW) and alkaline water with glutathione and vitamin C (Vit C) is expected the organism to act preventively to hyperthermic stress (HS). The aim of our research is to determine the influence of AW by adding enzymatic and non-enzymatic antioxidants, glutathione and Vit C during the HS. This treatment led to preventive action of the body from the influence of HS. All experiments were performed on female Wistar rats, divided into three groups of 15 individuals. Oxidative stress was caused by acute hyperthermic exposure. The first group is a control group, the second group is treated with AW, and the third group AW with added glutathione and Vit C. The duration of treatment lasted 21 days. In all rats, the body temperature (BT) was measured with a thermometer from 0-80 minutes, during exposure with a high ambient temperature of up to 41 ° C. The first group has a higher elevated BT of the other two groups. In the second group we have a BT increase, but with a lower value of the first group for 0.6 ° C and slightly higher than the third group for 0.4 ° C. In the third group we also have elevated BT values, but with lower values of the first group for 0.8 ° C and from the second group with lower values of BT for 0.4 ° C. Groups of rats treated AW and AW with added glutathione and Vit C react with slightly lower values of risen BT than the control group. We have received data that AW, glutathione and Vit C have an impact on the organism in the emergence of resistance to HS.

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