THE EFFECT OF SINAPIS ESSENTIAL OIL ON CHICKEN EMBRYO DEVELOPMENT: A COMPARATIVE EXPERIMENTAL STUDY
Bardhyl LIMANI, Bardha IBISHI, Zejndeije ADEMI, Hirijete IDRIZI, Hazir POLLOZHANI
Abstract
This study investigates the effect of Sinapis essential oil (Brassica juncea) on the embryonic development of chickens. Known for its antimicrobial, antioxidant, and growth-stimulating properties, Sinapis essential oil was selected to explore its potential influence during embryogenesis. Thirty fertilized chicken eggs were divided into two equal groups: 15 eggs in the control group (untreated), and 15 eggs in the experimental group, which received 0.05 ml (1.5%)of Sinapis essential oil injected into the air cell on day 1 of incubation.
Embryonic development was monitored on days 7, 14, and 21. Measurements included egg weight (recorded weekly), embryo weight, length, width, and eye size, along with visual assessment of organ differentiation. Results showed embryos in the treated group had superior growth: embryo weight increased by 1–1.5 g, average length reached 3.0 cm (vs. 2.5 cm), width 2.4 cm (vs. 1.8 cm), and eye diameter 0.7 mm (vs. 0.5 mm) compared to controls. Organs were visibly more defined in the treated group.
Data analysis using SPSS Two-way ANOVA, confirming statistically significant differences (p < 0.05) between groups. These findings suggest that Sinapis essential oil may positively influence embryo development in avian species.
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