THE IMPACT OF IONIZED WATER COMBINED WITH ADDITIONAL NON-ENZYMATIC ANTIOXIDANTS ON THE CONCENTRATION OF CREATININE IN RATS' SERUM DURING HYPERTHERMIC STRESS

Majlinda ADEMI

Abstract

One of the most important factors that could promote the production of reactive oxygen species (ROS) is hyperthermic stress. The cellular redox state has been found to be negatively impacted by elevated levels of reactive oxygen species (ROS). Ionized water or Electrolyzed Reduced Water (ERW) has been proven in numerous studies to have the ability to scavenge free radicals that are formed by hydrogen molecules with a substantial reducing capacity and may be involved in the regulation of cellular redox. The goal of our study was to see the impact of ionized water on the concentration of creatinine in rats' serum during hyperthermic stress by adding other non-enzymatic antioxidants, glutathione, and vitamin C. White Wistar laboratory rats, female, weighing 180–220 g, young rats, separated into three groups of 15, were used for the experiment. Oxidative stress was induced by acute hyperthermic exposure to 41°C. The first group was a control group (CPM) treated with natural water, the second group was treated with ionized water (TAM), and the third group was treated with ionized water with added glutathione and vitamin C (TAD). The duration of treatment lasted 21 days. Acute heat stress, except that it results in oxidative stress, conditions accelerated catabolic reactions in the body. The higher concentration of urea and creatinine in the period of hyperthermic exposure is due to the intensified breakdown of proteins. In such conditions, urea and creatinine concentrations do not represent a consequence of the applied treatment.

Pages: 244 - 249