THE CONCENTRATION OF CHLORHEXIDINE GLUCONATE FROM THE PERIO-CHIP IN GINGIVAL FLUID IN PATIENTS WITH PERIODONTAL DISEASE

Sahmedin SALI, Liburn KURTISHI, Lindim POSHKA

Abstract

Purpose: To determine the concentration of chlorhexidine gluconate (CHX) from the Perio-chip in cervical gingival fluid (FCG) at different time intervals in patients with clinical manifestation of the second stage of periodontal disease. Material and method: A total of 22 patients of both sexes aged 40-65 years of age, who were diagnosed with periodontal disease of the second clinical stage were followed up. The researchers who were part of the research had registered the periodontal pocket depth of 5 mm. Perio-chip was applied to them, where the concentration of chlorhexidine gluconate was measured at certain time intervals (after 1, 24, 48, 96, 168 hours after the application). The determination of chlorhexidine was carried out through two procedures: the extraction of gingival fluid and the quantitative determination of chlorhexidine gluconate in it. Extraction of gingival fluid: For this purpose was used Whatmann filter paper no. 5 with dimensions of 2x6 mm with which the part determined for measurement is inserted into the periodontal pocket 1 mm, where the liquid is drawn through the Whatmann filter paper through the absorption forces against the capillaries through diffusion. The strips taken for determining the concentration of chlorhexidine gluconate in the gingival fluid with the HPLC method, the analyzes at different time intervals for the concentration of chlorhexidine gluconate were carried out at the Institute for Chemical Application and Pharmaceutical Analysis at the Faculty of Pharmacy in Skopje. Results: The highest concentration was reached in the 1st hour after the application of the Perio-chip, while the concentration within 7 days moved from the values ??of 1148.70-624.37 µg mL-1where it is contained after the application of the medicinal asset. After 2-4 days certain concentration reaches the value up to 1300 µg mL-1, while after this time interval the concentration gradually decreases but remains at values ??above 125 µg mL-1 Conclusion: According to this formulation we can calculate that this formulation has an effective controlled continuous release system of chlorhexidine gluconate.

Pages: 320 - 324